Biopsy specimens were stained for eosinophil cationic protein to enumerate eosinophils within the airway mucosa, as previously referred to. Nucleated cellular material staining for Ki67 were counted within the epithelium and the lamina propria as individual compartments . Statistical Analysis Characteristics of the subjects and spirometric outcomes were summarized by using descriptive statistics, and between-group variations were assessed through one-way analysis of variance. Paired assessment of data acquired within organizations before and after problem was performed by using the Wilcoxon test.We assumed a baseline LVESVI of 100 ml per square meter, improvements of 20 ml per square meter in the restoration group and 35 ml per square meter in the replacement unit group, and equal 1-year mortality of 10 to 20 percent in the two groupings.16,17 The principal null hypothesis was that there will be no between-group difference in the LVESVI at 12 months. We tested this hypothesis in an intention-to-treat analysis utilizing a two-tailed Wilcoxon rank-sum test, at a 0.05 alpha level.